Abstract:

A method for high performance liquid chromatography- mass spectrometry (HPLC-MS) determination of carbendazim, thiabendazole, thiophanate-methyl and prochloraz residues in vegetables and fruits was established. The sample was extracted with methanol-HCl aqueous as extraction solvents, and then cleaned up with solid-phase extraction. After separated by reversed-phase liquid chromatography, it was detected by a mass detector. The detectable limits of antistaling agents are 1.0 μg/kg (carbendazim), 2.0 μg/kg (thiabendazole and prochloraz), 5.0 μg/kg (thiophanate-methyl). The recovery of carbendazim is 92.8% to 103.2% with the RSD of 2.27% to 4.01%, the recovery of thiabendazole is 90.9% to 102.5% with the RSD of 2.44% to 4.17%, the recovery of thiophanate-methyl is 74.9% to 99.2% with the RSD of 2.97% to 4.55%, and the recovery of prochloraz is 81.9% to 97.6% with the RSD of 2.64% to 4.49%.

Key words: vegetables, fruits, antistaling agents, residue, HPLC-MS